Donors leukocytes present in cellular blood components are
linked to wide range of transfusion adverse reactions mainly
non-hemolytic reactions, HLA alloimmunization and platelet
refractoriness.
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Techniques for preparing leukocytes reduced components are:
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New Semi-automated method (using Top and Bottom quadruple
bags and OPTI System)
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Techniques for Preparing Platelet concentrates in this blood
bank are :
- from platelet rich plasma-platelet yield is 5x1010
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from Buffy coat - platelet yield is 6-7X 1010 with
leukocytes reduced to log 3, 70-99% leuko-reduction
Platelets are preserved in the bags of PVC plasticized with
trimalliate (Baxter PL 1240) for 5 days at 20-240C in
incubator with agitation.